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유경재 님께서 남기신 글 (2004-03-20 12:27:17, Hit : 8264)
SUMOlyated proteins?

: J Biol Chem. 2004 Mar 11 [Epub ahead of print] Links

  
Broad-spectrum Identification of cellular SUMO substrate proteins.

Zhao Y, Kwon SW, Anselmo A, Kaur K, White MA.

Cell Biology, UT Southwestern medical Center, Dallas, TX 75390.

Reversible covalent modification of proteins with SUMO (small ubiquitin-related modifier) is emerging as an important system contributing to dynamic regulation of protein function. To enhance our understanding of the cell regulatory systems impacted by sumoylation, we are using affinity chromatography coupled HPLC/MS/MS for unbiased identification of candidate cellular SUMO substrate proteins. Here we describe the identification of 21 candidate sumoylated proteins from whole-cell lysates of HEK293 cells. The nature of the proteins identified is consistent with a role for sumoylation in diverse cell regulatory systems, but highlights regulation of chromatin organization and gene expression as major systems targeted by the sumoylation machinery.

PMID: 15016812 [PubMed - as supplied by publisher]

-----------------------------------------------------------------------------------------------
TABLE 1. SUMO targets
Identified proteins MW [kDa]# of peptides ID NCBI #
Sumoylation machinery
RanBP2 * 357.9 6 NP_006258
SUMO-1 E1 C subunit 71.2 5 AAD12784
SUMO-1 E1 N subunit 38.4 8 NP_005491
Sumo E2I 20.4 5 AAH00744
Sumo 2 14.7 10 XP_212611
Sumo 1 11.5 16 NP_003343
Chromatin Associated Factors
PML* 90.6 2 NP_150242
TIF1-beta 88.5 2 NP_005753
TFII-I 110 4 NP_127494
NXP-2 108.2 1 Q14149
zinc finger protein 262 136.2 2 NP_005086
Dna Topoisomerase I * 66.6 2 1LPQ_A
BTB-like domain proteins
similar to MGC25497 83.4 1 NP_945342
MGC2628 26.5 3 NP_076981
RNA Polymerases
RNA Pol III subunit 155kD 155.6 3 NP_008986
RNA Pol III subunit RPC2 127.6 3 NP_060552
RNA Pol III subunit RPC5 77.4 2 BAA95976
RNA Pol III subunit 62kD 60.6 2 NP_006459
RNA Pol III RPC8 19.6 1 AAH17248
RNA Pol I subunit 38.6 1 NP_004866
RNA Pol II subunit RPB5 24.5 1 AAC03238
Other
RanGAP1* 67.3 8 BAB47464
HSP70.1 70 4 P08107
ERp72 72.9 4 NP_004902
ErbB3 147.8 1 NP_001973
polymyositis/scleroderma autoantigen 2 100.7 1 XP_001527
NOP5 52.4 2 AAF29084
* indicates previously indentified SUMO
target.

TABLE 2. Consensus SUMO Target Sites
Identified proteins (A,V,L,I,F,W,M) K X E
TIF1-beta VKEE, IKEE
TFII-I VKTE, VKEE, VKEE, IKQE
NXP-2 IKAE, MKSE, VKKE, VKKE, VKAE,
IKTE
zinc finger protein 262 AKEE, AKSE
similar to MGC25497 VKSE, IKQE
MGC2628 None
RNA Pol III subunit 155kD AKAE, AKYE
RNA Pol III subunit RPC2 None
RNA Pol III subunit RPC5 AKLE, IKEE
RNA Pol III subunit 62kD None
RNA Pol III RPC8 None
RNA Pol I subunit None
RNA Pol II subunit RPB5 None
HSP70.1 None
ERp72 VKEE, FKGE, VKFE
ErbB3 IKRE
polymyositis/scleroderma autoantigen 2 AKSE, IKSE
NOP5 AKLE, AKTE, VKVE, IKEE


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  2004/03/20 
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